Anatoli N. Lopatin
Associate Professor of Molecular and Integrative Physiology
7812 MS II, Box 5622
Tel: 615-8903; E-mail: alopatin@umich.edu
Education
1976-1982
M.S.
Physics of Living Systems. Moscow Institute of Physics and
Technology,
1986-1990
Ph.D.
Biological Sciences.
Title: “Potassium and Chloride Channels in Pheochromocytoma
PC12”. Advisor: Dr. EI Melnik
Postdoctoral Training
1990-1992
Postdoctoral Fellow
Department of Neurobiology,
Physiology and biophysics of ion channels.
1992-1997
Postdoctoral Fellow
Department of Cell Biology and Physiology,
Physiology and biophysics of ion channels.
Academic Appointments
1997-2000
Research Assistant Professor
Department of Cell Biology and Physiology,
2000-2008
Assistant Professor
Department of Molecular and Integrative Physiology,
2008-present
Associate Professor
Department of Molecular and Integrative Physiology,
Teaching Activities
2015 Physiology 577: Membrane and Cell Physiology (Winter Semester); Course Director
DENT 638 Cardiovascular Systems (Winter Semester)
Physiology 592: Integrative Neuroscience (Winter Semester)
Bibliography
Peer-Reviewed
Manuscripts
1. Bendukidze ZA, Lopatin AN,
Ignatjeva VB and Sokolova SN.
Method of isolating single guinea-pig cardiomyocytes using pronase -
role of calcium-ions. Bulletin
of Experimental Biology and Medicine (Russ),
1985; 99(6):
833-836
2.
Lopatin AN, Kondratjev AD, Loginov BV and Melnik EI.
Potassium channels of clonal line pheochromocytoma.
Biologicheskie Membrany
(Russ), 1988; 5(7): 711-717
3. Lopatin AN, Melnik EI, Posypanova GA, Kondratjev AD and
Severin SE. Sodium currents in differentiating PC12 cells:the effect
of longterm incubation with serotonin. Biologicheskie Membrany
(Russ),
1989;
6(4): 428-430
4. Lopatin AN, Kondratjev AD and Melnik EI. Anion channels of
pheochromocytoma PC12s: the action of colchicine. Biologicheskie
Membrany (Russ),
1990; 7(2): 205-207
5. Kiselev
MF, Melnik EI, Vorontsov EA, Kondratyev AD, Kuznetsov SL,
Lopatin AN, Kryukov LN.
Effect of fluoro-containing esters of
3-(2,2-Dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid on the
kinetics of inactivation of sodium current in neuroblastoma
(neuro-2a) cells.
Bioorganicheskaya Khimiya
(Russ), 1991; 17(1): 136-140
6. Lopatin AN and Nichols CG. 2,3-Butanedione Monoxime (BDM)
inhibition of delayed rectifier (DRK1) Potassium channels expressed
in Xenopus oocytes. Journal of Pharmacology and Experimental
Therapeutics, 1993; 265: 1011-1016
7. Nichols CG and Lopatin AN. Trypsin and α-chymotrypsin
treatment abolishes glibenclamide sensitivity of KATP channels in
rat ventricular myocytes. Pflugers Archive (European Journal of
Physiology), 1993;
422: 617-619
8. Lopatin AN and Nichols CG. Block of delayed rectifier
(DRK1) K+ channels by internal 2,3-butanedione monoxime
in Xenopus oocytes. Receptors and Channels,
1993;
1: 279-286
9. Lopatin AN and Nichols CG. Internal Na+ and Mg2+
blockade of DRK1 (Kv2.1) potassium channels expressed in
Xenopus oocytes. Inward rectification of a delayed rectifier.
Journal of General Physiology,
1994;
103: 203-216
10. Makhina EN, Kelly AJ, Lopatin AN, Mercer RW and Nichols
CG. Cloning and expression of a novel inward rectifier potassium
channel from human brain. Journal of Biological Chemistry,
1994;
269: 20468-20474
11. Lopatin AN, Makhina EN and Nichols CG. Potassium channel
block by cytoplasmic polyamines as the mechanism of intrinsic
rectification. Nature,
1994;
372: 366-369
12. Lopatin AN
and Nichols CG. The mechanism of
inward rectification of potassium channels. Journal of General
Physiology, 1995;
106: 923-955
13.
Nichols CG, Lopatin AN, Makhina EN, Pearson WL and Sha Q.
Inward rectification and implications for cardiac excitability.
Circulation Research,
1996; 78: 1-7
14. Sha Q, Romano C, Lopatin AN and Nichols CG. Spermidine
release from Xenopus
oocytes: Electrodiffusion through a membrane channel. Journal of
Biological Chemistry,
1996; 271: 3392-3397
15.
Lopatin AN and
Nichols CG.
16.
Lopatin AN and
Nichols CG.
17.
Shyng SL, Sha Q, Ferrigni T, Lopatin AN
and Nichols CG. Depletion of
intracellular polyamines relieves inward rectification of potassium
channels. Proceedings of National
18.
Cohen NA, Sha Q, Makhina EN, Lopatin AN,
Linder ME, Snyder SH and Nichols CG. Inhibition of an inward
rectifier potassium channel by
G-protein βγ subunits. Journal of Biological Chemistry,
1996;
271: 32301-32305
19.
Lopatin AN,
Makhina EN and Nichols CG. A novel crystallization method for
visualizing the membrane localization of potassium channels.
Biophysical Journal, 1998;
74: 2159-2170
20. Lopatin
AN,
Shantz LM, Mackintosh CA, Nichols CG and Pegg AE. Modulation of
Potassium Channels in the Hearts of Transgenic and Mutant Mice with
Altered Polyamine Biosynthesis. Journal of Molecular and Cellular
Cardiology, 2000;
32(11): 2007-2024
21.
McLerie M and Lopatin AN. Dominant negative suppression of IK1
in the mouse heart leads to altered cardiac excitability.
Journal of Molecular and Cellular Cardiology,
2003;
35(4): 367-378
This paper is highlighted in the Editorial. When less is more: what
genetic manipulation tells us about cardiac I(K1). Journal of
Molecular and Cellular Cardiology, 2003; 35(4): 343-345
22. Flagg TP, Charpentier F, Manning-Fox J, Remedi MS, Enkvetchakul
D, Lopatin A, Koster J and Nichols C. Remodeling of
excitation-contraction coupling in transgenic mice expressing
ATP-insensitive sarcolemmal KATP channels. American
Journal of Physiology. Heart and Circulation Physiology,
2004; 286(4): H1361-H1369
23.
Li J, McLerie M and Lopatin AN. Transgenic up-regulation of IK1
in the mouse heart leads to multiple abnormalities of cardiac
excitability. American Journal of Physiology. Heart and
Circulation Physiology,
2004; 287(6): H2790-H2802
24. Flagg TP, Remedi MS, Masia R, Gomes J, McLerie M,
Lopatin AN and Nichols CG. Transgenic overexpression of SUR1 in the
heart suppresses sarcolemmal KATP.
Journal of Molecular and Cellular Cardiology,
2005; 39(4): 647-656
25.
Panama BK and Lopatin AN.
Differential polyamine sensitivity in Kir2 channels.
Journal of Physiology, 2006;
571(2): 287-302
26. Dobrzynski H, Billeter R, Greener ID, Tellez JO, Chandler NJ,
Flagg TP, Nichols CG, Lopatin
AN and Boyett MR. Expression of Kir2.1 and Kir6.2 transgenes
under the control of the alpha-MHC promoter in the sinoatrial and
atrioventricular nodes in transgenic mice.
Journal of Molecular and
Cellular Cardiology, 2006;
41: 855-867
27. Noujaim SF, Pandit SV, Berenfeld O, Vikstrom K, Cerrone M,
Mironov S, Zugermayr M,
Lopatin AN and Jalife J. Up-regulation of the inward rectifier K+
current (IK1) in the mouse heart accelerates and
stabilizes rotors. Journal of Physiology, 2007;
578: 315-326
28. Fu Y, Huang X, Piao L,
Lopatin AN, and Neubig RR. Endogenous RGS proteins modulate SA
and AV nodal functions in isolated heart: implications for sick
sinus syndrome and AV block. American Journal of Physiology.
Heart and Circulation Physiology,
2007; 292: H2532-H2539
29. Flagg TP, Patton B, Masia R,
30. Piao L, Li J, McLerie M and Lopatin AN. Transgenic upregulation of IK1 in the mouse heart is proarrhythmic. Basic Research in Cardiology, 2007; 102(5): 416-428
31. Piao L, Li J, McLerie M and
Lopatin AN. Cardiac IK1
channels underlie early action potential shortening during hypoxia
in the mouse heart. Journal of
Molecular and Cellular Cardiology,
2007;
43(1): 27-38
This paper is highlighted in the
Editorial. IK1 and cardiac hypoxia: after the long and
short QT syndromes, what else can go wrong with the inward rectifier
K+ currents? Journal of Molecular and Cellular
Cardiology, 2007; 43(1): 15-17
32. Lopez-Santiago LF, Meadows LS, Ernst SJ, Chen C, Malhotra JD,
McEwen DP, Speelman A, Noebels JL, Maier SK,
Lopatin AN, Isom LL.
Sodium channel Scn1b null mice exhibit prolonged QT and RR
intervals. Journal of
Molecular and Cellular Cardiology.
2007; 43: 636-47
33. Panama BK, McLerie M and
Lopatin AN. Heterogeneity of IK1 in the mouse heart.
American Journal of Physiology. Heart and Circulation Physiology. 2007; 293(6):H3558-67
34. Day SM, Coutu P, Wang W, Herron TJ, Turner I, Shillingford M,
Lacross NC, Converso KL, Piao L, Li J,
Lopatin AN, Metzger JM.
Cardiac-directed parvalbumin transgene expression in mice shows
marked heart rate dependence of delayed Ca2+ buffering
action. Physiological
Genomics. 2008; 33:
312-322
35. Chen J, Hessler JA, Putchakayala K, Panama BK, Khan DP, Hong S,
Mullen DG, Dimaggio SC, Som A, Tew GN,
Lopatin AN, Baker JR,
Holl MM, and Orr BG. Cationic nanoparticles induce nanoscale
disruption in living cell plasma membranes.
J Phys Chem B.
2009; 113: 11179-11185
36. Panama BK, McLerie M and
Lopatin AN. Functional Consequences of Kir2.1/Kir2.2 Subunit
Heteromerization. Pflugers
Archiv-European Journal of Physiology
2010;
460(5): 839.
37. Cheng LF, Wang F, Lopatin
AN. Metabolic stress in isolated mouse ventricular myocytes
leads to remodeling of t-tubules.
Am J Physiol Heart Circ
Physiol. 2011; 301:
H1984-H95.
Reviews (including peer reviewed)
1.
Nichols CG and Lopatin AN.
Inward rectifier
potassium channels Annual Review of Physiology,
1998;
59: 171-191
2. Lopatin AN, Makhina EN and Nichols CG. Novel tools for
localizing ion channels in living cells. Trends in
Pharmacological Sciences,
1998;
19: 395-398
This paper is highlighted on the
cover page of the same issue of the
Trends in Pharmacological Sciences
3. Lopatin AN and Nichols CG. Inward rectifiers in the heart:
An update on IK1. Journal of Molecular and Cellular
Cardiology, 2001;
33(4): 625-638
4. Lopatin AN Inward rectification and cardiac excitability.
Biological Membranes (Russ),
2002;
19(1): 57-65
5. Anumonwo JM and Lopatin AN.
Cardiac strong inward rectifier potassium channels.
J Mol Cell Cardiol.
2010; 48(1): 45-54
Book Chapters
1.
Nichols CG,
2.
Nichols CG and Lopatin AN. Inwardly Rectifying potassium
channels: mechanisms of rectification. In “Potassium Ion Channels.”
Eds. Y. Kurachi, L.Y. Lan and M. Lazduski. Academic Press. ICBN
0-12-153346-8. 1999
3.
Shyng SL, Lopatin AN and Nichols CG. Molecular Biology of
Inward Rectifier and ATP-sensitive K+ channels. In
“Potassium Channels in Cardiovascular Biology.
Molecular Biology of
potassium channels.” Eds. S.L. Archer and N.J. Rush. Kluwer
Academic/Plenum Publishers. ISBN 0-306-46402-0.
2000
4.
Lopatin AN,
Makhina EN and Nichols CG.
5.
Lopatin AN
and Nichols CG. Inward rectifier potassium channels. In
“Encyclopedic Reference of Molecular Pharmacology”, Eds. S.
Offermanns and W. Rosenthal. Springer Verlag,
6.
Lopatin AN
and Nichols CG. Inward rectifier potassium channels. In
“Encyclopedic Reference of Molecular Pharmacology”, Eds. S.
Offermanns and W. Rosenthal. Springer Verlag,
Books Edited
Lopatin AN and Nichols CG. “Ion Channel Localization Methods and Protocols” in series of “Methods in Pharmacology and Toxicology”. Humana Press. ISBN: 0-89603-8333-5. 2001