7812 MS II, 1301 E. Catherine St.
Ann Arbor, MI 48109-5622
734-615-8903
alopatin@umich.edu
Welcome to Anatoli's page!

 

 

Anatoli N. Lopatin

Associate Professor of Molecular and Integrative Physiology

7812 MS II, Box 5622

Tel: 615-8903; E-mail: alopatin@umich.edu

 

Education

   1976-1982                M.S.

                                    Physics of Living Systems. Moscow Institute of Physics and Technology,    Moscow, Russia

 

   1986-1990                Ph.D.

                                    Biological Sciences. Research Center of Molecular Diagnostics, Moscow,  Russia

                                    Title: “Potassium and Chloride Channels in Pheochromocytoma PC12”. Advisor: Dr. EI Melnik

 

Postdoctoral Training

   1990-1992                Postdoctoral Fellow

                                    Department of Neurobiology, Research Center of Molecular Diagnostics, Moscow, Russia

                                    Physiology and biophysics of ion channels. Mentor: Dr. EI Melnik

 

   1992-1997                Postdoctoral Fellow

                                    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO

                                    Physiology and biophysics of ion channels. Mentor: Dr. CG Nichols

 

Academic Appointments

   1997-2000                Research Assistant Professor

                                    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO

 

   2000-2008                Assistant Professor

                                     Department of Molecular and Integrative Physiology, University of  Michigan, Ann Arbor, MI

 

   2008-present             Associate Professor

                                     Department of Molecular and Integrative Physiology, University of   Michigan, Ann Arbor, MI

 

Teaching Activities

2015                            Physiology 577: Membrane and Cell Physiology (Winter Semester); Course Director

                                    DENT 638 Cardiovascular Systems (Winter Semester)

                                     Physiology 592: Integrative Neuroscience (Winter Semester)

 

Bibliography

   Peer-Reviewed Manuscripts

1. Bendukidze ZA, Lopatin AN, Ignatjeva VB and Sokolova SN. Method of isolating single guinea-pig cardiomyocytes using pronase - role of calcium-ions. Bulletin of Experimental Biology and Medicine (Russ), 1985; 99(6): 833-836

2. Lopatin AN, Kondratjev AD, Loginov BV and Melnik EI. Potassium channels of clonal line pheochromocytoma. Biologicheskie Membrany (Russ), 1988; 5(7): 711-717

3. Lopatin AN, Melnik EI, Posypanova GA, Kondratjev AD and Severin SE. Sodium currents in differentiating PC12 cells:the effect of longterm incubation with serotonin. Biologicheskie Membrany (Russ), 1989; 6(4): 428-430

4. Lopatin AN, Kondratjev AD and Melnik EI. Anion channels of pheochromocytoma PC12s: the action of colchicine. Biologicheskie Membrany (Russ), 1990; 7(2): 205-207

5. Kiselev MF, Melnik EI, Vorontsov EA, Kondratyev AD, Kuznetsov SL, Lopatin AN, Kryukov LN. Effect of fluoro-containing esters of 3-(2,2-Dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid on the kinetics of inactivation of sodium current in neuroblastoma (neuro-2a) cells. Bioorganicheskaya Khimiya (Russ), 1991; 17(1): 136-140

6. Lopatin AN and Nichols CG. 2,3-Butanedione Monoxime (BDM) inhibition of delayed rectifier (DRK1) Potassium channels expressed in Xenopus oocytes. Journal of Pharmacology and Experimental Therapeutics, 1993; 265: 1011-1016

7. Nichols CG and Lopatin AN. Trypsin and α-chymotrypsin treatment abolishes glibenclamide sensitivity of KATP channels in rat ventricular myocytes. Pflugers Archive (European Journal of Physiology), 1993; 422: 617-619

8. Lopatin AN and Nichols CG. Block of delayed rectifier (DRK1) K+ channels by internal 2,3-butanedione monoxime in Xenopus oocytes. Receptors and Channels, 1993; 1: 279-286

9. Lopatin AN and Nichols CG. Internal Na+ and Mg2+ blockade of DRK1 (Kv2.1) potassium channels expressed in Xenopus oocytes. Inward rectification of a delayed rectifier. Journal of General Physiology, 1994; 103: 203-216

10. Makhina EN, Kelly AJ, Lopatin AN, Mercer RW and Nichols CG. Cloning and expression of a novel inward rectifier potassium channel from human brain. Journal of Biological Chemistry, 1994; 269: 20468-20474

11. Lopatin AN, Makhina EN and Nichols CG. Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification. Nature, 1994; 372: 366-369

12. Lopatin AN and Nichols CG. The mechanism of inward rectification of potassium channels. Journal of General Physiology, 1995; 106: 923-955

13. Nichols CG, Lopatin AN, Makhina EN, Pearson WL and Sha Q. Inward rectification and implications for cardiac excitability. Circulation Research, 1996; 78: 1-7

14. Sha Q, Romano C, Lopatin AN and Nichols CG. Spermidine release from Xenopus oocytes: Electrodiffusion through a membrane channel. Journal of Biological Chemistry, 1996; 271: 3392-3397

15. Lopatin AN and Nichols CG. [K+]-dependence of open channel conductance in cloned inward rectifier potassium channels (IRK1, Kir2.1) Biophysical Journal, 1996a; 71: 682-694

16. Lopatin AN and Nichols CG. [K+]-dependence of polyamine induced rectification in inward rectifier potassium channels (IRK1, Kir2.1). Journal of General Physiology, 1996b; 108: 105-113

17. Shyng SL, Sha Q, Ferrigni T, Lopatin AN and Nichols CG. Depletion of intracellular polyamines relieves inward rectification of potassium channels. Proceedings of National Academy of Science, USA, 1996; 93: 12014-12019

18. Cohen NA, Sha Q, Makhina EN, Lopatin AN, Linder ME, Snyder SH and Nichols CG. Inhibition of an inward rectifier potassium channel by G-protein βγ subunits. Journal of Biological Chemistry, 1996; 271: 32301-32305

19. Lopatin AN, Makhina EN and Nichols CG. A novel crystallization method for visualizing the membrane localization of potassium channels. Biophysical Journal, 1998; 74: 2159-2170

20. Lopatin AN, Shantz LM, Mackintosh CA, Nichols CG and Pegg AE. Modulation of Potassium Channels in the Hearts of Transgenic and Mutant Mice with Altered Polyamine Biosynthesis. Journal of Molecular and Cellular Cardiology, 2000; 32(11): 2007-2024

21. McLerie M and Lopatin AN. Dominant negative suppression of IK1 in the mouse heart leads to altered cardiac excitability. Journal of Molecular and Cellular Cardiology, 2003; 35(4): 367-378

This paper is highlighted in the Editorial. When less is more: what genetic manipulation tells us about cardiac I(K1). Journal of Molecular and Cellular Cardiology, 2003; 35(4): 343-345

22. Flagg TP, Charpentier F, Manning-Fox J, Remedi MS, Enkvetchakul D, Lopatin A, Koster J and Nichols C. Remodeling of excitation-contraction coupling in transgenic mice expressing ATP-insensitive sarcolemmal KATP channels. American Journal of Physiology. Heart and Circulation Physiology, 2004; 286(4): H1361-H1369

23. Li J, McLerie M and Lopatin AN. Transgenic up-regulation of IK1 in the mouse heart leads to multiple abnormalities of cardiac excitability. American Journal of Physiology. Heart and Circulation Physiology, 2004; 287(6): H2790-H2802

24. Flagg TP, Remedi MS, Masia R, Gomes J, McLerie M, Lopatin AN and Nichols CG. Transgenic overexpression of SUR1 in the heart suppresses sarcolemmal KATP. Journal of Molecular and Cellular Cardiology, 2005; 39(4): 647-656

25. Panama BK and Lopatin AN. Differential polyamine sensitivity in Kir2 channels. Journal of Physiology, 2006; 571(2): 287-302

26. Dobrzynski H, Billeter R, Greener ID, Tellez JO, Chandler NJ, Flagg TP, Nichols CG, Lopatin AN and Boyett MR. Expression of Kir2.1 and Kir6.2 transgenes under the control of the alpha-MHC promoter in the sinoatrial and atrioventricular nodes in transgenic mice. Journal of Molecular and Cellular Cardiology, 2006; 41: 855-867

27. Noujaim SF, Pandit SV, Berenfeld O, Vikstrom K, Cerrone M, Mironov S, Zugermayr M, Lopatin AN and Jalife J. Up-regulation of the inward rectifier K+ current (IK1) in the mouse heart accelerates and stabilizes rotors. Journal of Physiology, 2007; 578: 315-326

28. Fu Y, Huang X, Piao L, Lopatin AN, and Neubig RR. Endogenous RGS proteins modulate SA and AV nodal functions in isolated heart: implications for sick sinus syndrome and AV block. American Journal of Physiology. Heart and Circulation Physiology, 2007; 292: H2532-H2539

29. Flagg TP, Patton B, Masia R, Mansfield C, Lopatin AN, Yamada KA and Nichols CG. Arrhythmia susceptibility and premature death in transgenic mice overexpressing both SUR1 and Kir6.2[ΔN30,K185Q] in the heart. American Journal of Physiology. Heart and Circulation Physiology, 2007; 293(1): H836-845

30. Piao L, Li J, McLerie M and Lopatin AN. Transgenic upregulation of IK1 in the mouse heart is proarrhythmic. Basic Research in Cardiology, 2007; 102(5): 416-428

31. Piao L, Li J, McLerie M and Lopatin AN. Cardiac IK1 channels underlie early action potential shortening during hypoxia in the mouse heart. Journal of Molecular and Cellular Cardiology, 2007; 43(1): 27-38

This paper is highlighted in the Editorial. IK1 and cardiac hypoxia: after the long and short QT syndromes, what else can go wrong with the inward rectifier K+ currents? Journal of Molecular and Cellular Cardiology, 2007; 43(1): 15-17

32. Lopez-Santiago LF, Meadows LS, Ernst SJ, Chen C, Malhotra JD, McEwen DP, Speelman A, Noebels JL, Maier SK, Lopatin AN, Isom LL. Sodium channel Scn1b null mice exhibit prolonged QT and RR intervals. Journal of Molecular and Cellular Cardiology. 2007; 43: 636-47

33. Panama BK, McLerie M and Lopatin AN. Heterogeneity of IK1 in the mouse heart. American Journal of Physiology. Heart and Circulation Physiology. 2007; 293(6):H3558-67

34. Day SM, Coutu P, Wang W, Herron TJ, Turner I, Shillingford M, Lacross NC, Converso KL, Piao L, Li J, Lopatin AN, Metzger JM. Cardiac-directed parvalbumin transgene expression in mice shows marked heart rate dependence of delayed Ca2+ buffering action. Physiological Genomics. 2008; 33: 312-322

35. Chen J, Hessler JA, Putchakayala K, Panama BK, Khan DP, Hong S, Mullen DG, Dimaggio SC, Som A, Tew GN, Lopatin AN, Baker JR, Holl MM, and Orr BG. Cationic nanoparticles induce nanoscale disruption in living cell plasma membranes. J Phys Chem B. 2009; 113: 11179-11185

36. Panama BK, McLerie M and Lopatin AN. Functional Consequences of Kir2.1/Kir2.2 Subunit Heteromerization. Pflugers Archiv-European Journal of Physiology 2010; 460(5): 839.

37. Cheng LF, Wang F, Lopatin AN. Metabolic stress in isolated mouse ventricular myocytes leads to remodeling of t-tubules. Am J Physiol Heart Circ Physiol. 2011; 301: H1984-H95.

38. Moench I, Meekhof KE, Cheng LF and Lopatin AN. Resolution of hypo-osmotic stress in isolated mouse ventricular myocytes causes sealing of t-tubules. Experimental Physiology. 2013; 98: 1164-77. PMCID: PMC3746342. PMID: 23585327.

39. Moench I and Lopatin AN. Ca2+ homeostasis in sealed t-tubules of mouse ventricular myocytes. Journal of Molecular and Cellular Cardiology. 2014 Jul; 72:374-83; PMID: 24787472

 

   Reviews (including peer reviewed)

1. Nichols CG and Lopatin AN. Inward rectifier potassium channels Annual Review of Physiology, 1998; 59: 171-191

2. Lopatin AN, Makhina EN and Nichols CG. Novel tools for localizing ion channels in living cells. Trends in Pharmacological Sciences, 1998; 19: 395-398

This paper is highlighted on the cover page of the same issue of the Trends in Pharmacological Sciences

3. Lopatin AN and Nichols CG. Inward rectifiers in the heart: An update on IK1. Journal of Molecular and Cellular Cardiology, 2001; 33(4): 625-638

4. Lopatin AN Inward rectification and cardiac excitability. Biological Membranes (Russ), 2002; 19(1): 57-65

5. Anumonwo JM and Lopatin AN. Cardiac strong inward rectifier potassium channels. J Mol Cell Cardiol. 2010; 48(1): 45-54

 

   Book Chapters

1. Nichols CG, Cannell MB and Lopatin AN. Cell-free ion channel recording. In "Patch clamp techniques and Protocols.” Eds. A.A. Boulton, G.B. Baker and W. Walz. Humana Press Inc. Totowa, NJ, USA. 1994

2. Nichols CG and Lopatin AN. Inwardly Rectifying potassium channels: mechanisms of rectification. In “Potassium Ion Channels.” Eds. Y. Kurachi, L.Y. Lan and M. Lazduski. Academic Press. ICBN 0-12-153346-8. 1999

3. Shyng SL, Lopatin AN and Nichols CG. Molecular Biology of Inward Rectifier and ATP-sensitive K+ channels. In “Potassium Channels in Cardiovascular Biology. Molecular Biology of potassium channels.” Eds. S.L. Archer and N.J. Rush. Kluwer Academic/Plenum Publishers. ISBN 0-306-46402-0. 2000

4. Lopatin AN, Makhina EN and Nichols CG. Ch. 17. Crystallization technique for localizing ion channels in living cells. In: “Ion Channel Localization: Methods and Protocols.” Eds. Lopatin AN and Nichols CG. Humana Press Inc. Totowa, New Jersey. 2001

5. Lopatin AN and Nichols CG. Inward rectifier potassium channels. In “Encyclopedic Reference of Molecular Pharmacology”, Eds. S. Offermanns and W. Rosenthal. Springer Verlag, Berlin, Germany. 1st Ed. 2002

6. Lopatin AN and Nichols CG. Inward rectifier potassium channels. In “Encyclopedic Reference of Molecular Pharmacology”, Eds. S. Offermanns and W. Rosenthal. Springer Verlag, Berlin, Germany. 2nd Ed. 2007

 

   Books Edited

Lopatin AN and Nichols CG. “Ion Channel Localization Methods and Protocols” in series of “Methods in Pharmacology and Toxicology”. Humana Press. ISBN: 0-89603-8333-5. 2001